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Cloning and Expression
Transfection Kits and Reagents
Transfection Reagents
FuGENE 6 Transfection Reagent
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FuGENE 6 Transfection Reagent
FuGENE 6 Transfection Reagent
FuGENE 6 Transfection Reagent from Promega
(2)
Write a review
Citations:
(487)
Supplier Page
Supplier Page from
Promega for
FuGENE 6 Transfection Reagent
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Description
FuGENE 6 Transfection Reagent is a nonliposomal formulation designed to transfect plasmid DNA into a wide variety of cell lines with high efficiency and low toxicity
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
FuGENE 6 Transfection Reagent
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E2691
Quantity
1ml
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (487)
(1)
TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates
iScience
December 15, 2023
Rui Jiang, Wentao Zhu, Zhiwei Liao, Chunrong Yang, Jianguo Su
(IPTG),Sigma-Aldrich,Sigma-Aldrich,I6758 Hoechst 33342,AAT Bioquest,
AAT Bioquest
,
17530
FuGENE 6,Promega,
Promega
,
E2691
Restriction enzymes,Thermo Fisher Scientifc,Thermo Fisher Scientifc,N/A TLR7-LRR-GST,This paper,This
(2)
Anti-tau intrabodies: From anti-tau immunoglobulins to the development of functional scFv intrabodies
Molecular Therapy - Methods & Clinical Development
December 14, 2023
Dina Rodrigues Martins, Fern Sha, Wim Van der Elst, Pei-Yu Shih, Jens Devoght, Kristof Van Kolen, Marc Mercken, Bianca Van Broeck, Paul Declerck, Clara Theunis
medium was changed to DMEM without antibiotics prior to plasmid
DNA transfection
with FuGENE6 (
Promega
,
E2691
), following manufacturer’s instructions. At 48 h after
transfection
, culture medium was collected from
(3)
PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina
Nucleus (Austin, Tex.)
December 1, 2023
Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.
FuGENE 6
transfection
reagents (ThermoFisher Scientific/Invitrogen, cat#
L3000008
; and
Promega
, cat#
E2691
) were used for transient delivery of
PRR14
plasmids, in accordance with the manufacturer’s guidelines.
(4)
Direct and biologically significant interactions of human herpesvirus 8 interferon regulatory factor 1 with STAT3 and Janus kinase TYK2
PLOS Pathogens
November 20, 2023
Zunlin Yang, Qiwang Xiang, John Nicholas
(run at 12 V for ∼20 h). BAC16 genomes were
transfected
into iSLK cells by using Fugene 6 (
Promega
,
E2691
), and BAC16+ cells were selected by hygromycin (2.3 mM) treatment of cultures for 3 weeks.
Virus
was
(5)
High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation
Nature Methods
November 13, 2023
Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Theresa Wiesner, Damián Martínez, Tommaso Galgani, Louise Régnier, Aki Stubb,
et al.
(
356237
,
Corning
). Cells were seeded at 60% confluency.
Transfections
were performed using Fugene6 (
E2691
,
Promega
) according to the manufacturer’s instructions. Each coverslip was
transfected
with 750 ng of
(6)
Lysine Demethylase KDM2A Promotes Proteasomal Degradation of TCF/LEF Transcription Factors in a Neddylation-Dependent Manner
Cells
November 13, 2023
Tijana Šopin, František Liška, Tomáš Kučera, Dušan Cmarko, Tomáš Vacík
transfected
with plasmids (1 μg/1 cm of the dish diameter) using Fugene6 (
Promega
, Madison, WI, USA,
E2691
) or Turbofect (
ThermoFisher Scientific
, Waltham, MA, USA,
R0534
). HEK293T cells were treated with 1
(7)
Functional characterization of the CRY2 circadian clock component variant p.Ser420Phe revealed a new degradation pathway for CRY2
Journal of Biological Chemistry
November 10, 2023
Gizem Cagla Parlak, Ibrahim Baris, Seref Gul, Ibrahim Halil Kavakli
variant pMU2-P(CRY1)-(intron 336)-Cry2 using the FuGENE6
transfection reagent
(
Promega
; catalog no.:
E2691
) after 24 h, when they have reached 70% confluency. After 3 days of
incubation
, the cells were reset
(8)
Target gene regulatory network of miR-497 in angiosarcoma
bioRxiv
September 29, 2023
Annaleigh Benton, Emma Terwilliger, Noah M. Moriarty, Bozhi Liu, Ant Murphy, Hannah Maluvac, Mae Shu, Lauren E. Gartenhaus, Nimod D. Janson, Claire M. Pfeffer,
et al.
with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (
E2691
;
Promega
) and performing the
Dual-Luciferase Reporter Assay System
(
E1910
;
Promega
) after 72 h as previously
(9)
SIRT5 Activation and Inorganic Phosphate Binding Reduce Cancer Cell Vitality by Modulating Autophagy/Mitophagy and ROS
Antioxidants (Basel, Switzerland)
August 18, 2023
Barreca F, Aventaggiato M, Vitiello L, Sansone L, Russo MA, Mai A, Valente S, Tafani M.
plasmid (1 μg) was
transfected
into cells using FuGENE®
transfection reagent
(
Promega
, Milan, Italy;
E2691
) according to the manufacturer’s protocol. The day after, cells were transferred to a 100 mm dish. Three
(10)
Flat clathrin lattices are linked to metastatic potential in colorectal cancer
iScience
August 18, 2023
Cresens C, Solís-Fernández G, Tiwari A, Nuyts R, Hofkens J, Barderas R, Rocha S.
spun down. The
transfection
mixture for mEos3.2-
CLTA
was prepared by first adding FuGENE 6 (
Promega
,
E2691
) and then plasmid DNA to 100 μL DMEM with 1% GlutaMAX. For both methods the
transfection
mixture was
(11)
Depletion of SNORA33 Abolishes ψ of 28S-U4966 and Affects the Ribosome Translational Apparatus
Int J Mol Sci
August 8, 2023
Alzbeta Chabronova, Guus van den Akker, Bas A. C. Housmans, Marjolein M. J. Caron, Andy Cremers, Don A. M. Surtel, Mandy J. Peffers, Lodewijk W. van Rhijn, Virginie Marchand, Yuri Motorin,
et al.
respectively; sequences in Table S6). Plasmid DNA was
transfected
with Fugene6
Transfection Reagent
(
Promega
,
E2691
; Fugene:DNA 4:1, 0.5 μg DNA/well of 24-well plate). Cells were co-
transfected
with lacZ plasmid (10%
(12)
Chemogenetic regulation of the TARP-lipid interaction mimics LTP and reversibly modifies behavior
Cell Reports
August 1, 2023
Joongkyu Park, Coralie Berthoux, Erika Hoyos-Ramirez, Lili Shan, Megumi Morimoto-Tomita, Yixiang Wang, Pablo E. Castillo, Susumu Tomita
proteins","Chemicals, peptides, and recombinant proteins" ,,
FuGENE 6 transfection reagent
,
Promega
,Cat#
E2691
Doxycycline,Clontech,Cat# 631311
Tamoxifen
,
Sigma
,Cat#
T5648
; CAS: 10540–29-1 TOTO-3 iodide (642/660),
Invitrogen
,Cat#
(13)
Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice
Cell reports
July 25, 2023
Xing L, Simon JM, Ptacek TS, Yi JJ, Loo L, Mao H, Wolter JM, McCoy ES, Paranjape SR, Taylor-Blake B,
et al
.
pCIG empty vector or
UBE3A
-expressing constructs using 1 μL of Fugene6
transfection reagent
(
Promega
,
E2691
) mixed in Opti-
MEM
(
Invitrogen
). 24 h after
transfection
, BAR (
Firefly
) and Renilla luciferase activity
(14)
Super-resolution imaging unveils the self-replication of tau aggregates upon seeding
Cell reports
July 25, 2023
Dimou E, Katsinelos T, Meisl G, Tuck BJ, Keeling S, Smith AE, Hidari E, Lam JYL, Burke M, Lövestam S,
et al
.
L3000001
"
Penicillin-Streptomycin (10,000 U/mL
)",
Thermo Fisher Scientific
,Cat#
15140122
FuGENE 6,
Promega
,Cat#
E2691
MG132,Merck,Cat# M7449
Carfilzomib
(CFZ),
Cayman
Chemicals,Cat#
17554
; CAS: 868540-17-4 Rapamycin,
Merck
,Cat#
(15)
Phosphorylation by ATR triggers FANCD2 chromatin loading and activates the Fanconi anemia pathway
Cell reports
July 25, 2023
Kupculak M, Bai F, Luo Q, Yoshikawa Y, Lopez-Martinez D, Xu H, Uphoff S, Cohn MA.
and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, FuGENE6,
Promega
,Cat#
E2691
Lipofectamine 2000,
Invitrogen
,Cat#
11668019
Geneticin (G418 sulfate),
Thermo Fisher
,Cat#
10131-035
VE-821,Sigma-Aldrich,Cat#
(16)
Impaired ribosome-associated quality control of C9orf72 arginine-rich dipeptide-repeat proteins
Brain
July 3, 2023
Ashley P Viera Ortiz, Gregory Cajka, Olamide A Olatunji, Bailey Mikytuck, Ophir Shalem, Edward B Lee
plasmids (
CRISPR–Cas9
plasmid, EGFP and stalling reporters) was done using
Fugene 6 transfection reagent
(
E2691
,
Promega
) in a
1:3
ratio following the manufacturer’s recommendations. Cell lines were regularly tested
(17)
Adaptation of a transmitted/founder simian-human immunodeficiency virus for enhanced replication in rhesus macaques
PLOS Pathogens
July 3, 2023
Anya Bauer, Emily Lindemuth, Francesco Elia Marino, Ryan Krause, Jaimy Joy, Steffen S. Docken, Suvadip Mallick, Kevin McCormick, Clinton Holt, Ivelin Georgiev,
et al.
achieved by adding 6 μg of plasmid DNA in 18 microliters of
FuGENE 6 Transfection Reagent
(
Promega
#
E2691
); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded
(18)
SARS-CoV-2 Prevalence and Variant Surveillance among Cats in Pittsburgh, Pennsylvania, USA
Viruses
June 30, 2023
Santhamani Ramasamy, Abhinay Gontu, Sabarinath Neerukonda, Diana Ruggiero, Becky Morrow, Sheweta Gupta, Saranya Amirthalingam, John M. Hardham, Joshua T. Lizer, Michele Yon,
et al.
transfected
in HEK
293T cells
using FuGene6
transfection reagent
(
Promega
, Madison, WI, USA. Cat. No.
E2691
). The HEK
293T cells
were propagated using Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine
(19)
RBBP4 is an epigenetic barrier for the induced transition of pluripotent stem cells into totipotent 2C-like cells
Nucleic Acids Research
June 23, 2023
Wangfang Ping, Yingliang Sheng, Gongcheng Hu, Hongxin Zhong, Yaoyi Li, YanJiang Liu, Wei Luo, Chenghong Yan, Yulin Wen, Xinxiu Wang,
et al.
(Addgene, 92144) expressing a gRNA targeting the Tigre locus using FuGENE6
Transfection Reagent
(
Promega
,
E2691
) following the manufacturer's instructions.
Clones
were selected with 1 μg/ml puromycin for 3 days.
(20)
NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein
Proceedings of the National Academy of Sciences
June 13, 2023
Danica M. Sutherland, Michael Strebl, Melanie Koehler, Olivia L. Welsh, Xinzhe Yu, Liya Hu, Rita dos Santos Natividade, Jonathan J. Knowlton, Gwen M. Taylor, Rodolfo A. Moreno,
et al.
seeded into 24-well
tissue-culture
plates. Cells were
transfected
with cDNA using FuGene 6 (
Promega
,
E2691
) following the manufacturer’s instructions using a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega,
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Reviews
Write a Review
(1)
2/4/2020
Best Transfection Reagent for Large-Sized DNA
Sunita Meena
Good for large-sized DNA.
(1)
6/15/2016
Fugene 6 Transfection Kit
Benjamin Gerovac
This kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.
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FuGENE 6 Transfection Reagent from Promega
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